home *** CD-ROM | disk | FTP | other *** search
MacBinary | 1994-08-24 | 48.6 KB | [STAK/TRJT] |
open in:
MacOS 8.1
|
Win98
|
DOS
view JSON data
|
view as text
This file was processed as: MacBinary
(archive/macBinary).
You can browse this item here: Recipe Book
Confidence | Program | Detection | Match Type | Support
|
---|
100%
| dexvert
| HyperCard Stack (archive/hypercard)
| idMeta
| Supported |
10%
| dexvert
| MacBinary (archive/macBinary)
| fallback
| Supported |
100%
| file
| MacBinary II, inited, busy, Sat Apr 10 13:58:03 1993, modified Wed Aug 24 03:05:00 1994, creator 'TRJT', type HyperCard stack, 24576 bytes "Recipe Book" , at 0x6080 25014 bytes resource
| default (weak)
| |
99%
| file
| data
| default
| |
74%
| TrID
| Mac HyperCard stack (MacBinary)
| default
| |
25%
| TrID
| MacBinary 2
| default (weak)
| |
100%
| lsar
| MacBinary
| default
|
|
id metadata |
---|
key | value |
---|
macFileType | [STAK] |
macFileCreator | [TRJT] |
hex view+--------+-------------------------+-------------------------+--------+--------+
|00000000| 00 0b 52 65 63 69 70 65 | 20 42 6f 6f 6b 00 00 00 |..Recipe| Book...|
|00000010| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000020| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000030| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000040| 00 53 54 41 4b 54 52 4a | 54 05 00 00 00 00 00 00 |.STAKTRJ|T.......|
|00000050| 00 00 00 00 00 60 00 00 | 00 61 b6 a7 ec bb 2b aa |.....`..|.a....+.|
|00000060| 80 a1 9c 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000070| 00 00 00 00 00 00 00 00 | 00 00 81 81 a7 29 00 00 |........|.....)..|
|00000080| 00 00 08 00 53 54 41 4b | ff ff ff ff 00 00 00 00 |....STAK|........|
|00000090| 00 00 00 0a 00 00 60 00 | 00 00 08 00 00 00 00 00 |......`.|........|
|000000a0| 00 00 00 00 00 00 00 01 | 00 00 0a 2e 00 00 00 0a |........|........|
|000000b0| 00 00 0b a0 00 00 18 b0 | 00 00 00 02 00 00 02 60 |........|.......`|
|000000c0| 00 00 0e c4 00 00 00 00 | 00 05 00 00 50 00 00 00 |........|....P...|
|000000d0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000000e0| 02 10 80 00 02 20 80 00 | 02 20 80 00 02 20 80 00 |..... ..|. ... ..|
|000000f0| 9e 31 d4 67 00 00 00 04 | 00 35 00 40 01 8b 02 40 |.1.g....|.5.@...@|
|00000100| 00 00 00 00 01 90 02 80 | 00 00 00 00 00 00 00 00 |........|........|
|00000110| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000120| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000130| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000140| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000150| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000160| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000170| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000180| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000190| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000001a0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000001b0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000001c0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000001d0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000001e0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000001f0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000200| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000210| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000220| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000230| 00 00 0d 27 00 00 0c c5 | 01 56 02 00 00 00 00 00 |...'....|.V......|
|00000240| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000250| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000260| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000270| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000280| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000290| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000002a0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000002b0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000002c0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000002d0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000002e0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000002f0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000300| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000310| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000320| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000330| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000340| 00 00 00 00 00 00 00 00 | 80 00 00 00 08 00 00 00 |........|........|
|00000350| 88 00 22 00 88 00 22 00 | 88 88 22 22 88 88 22 22 |.."...".|..""..""|
|00000360| 88 aa 22 aa 88 aa 22 aa | cc aa 33 aa cc aa 33 aa |.."...".|..3...3.|
|00000370| ee aa bb aa ee aa bb aa | ee bb bb ee ee bb bb ee |........|........|
|00000380| ff bb ff ee ff bb ff ee | ff bb ff ff ff bb ff ff |........|........|
|00000390| 80 10 02 20 01 08 40 04 | ff ff ff ff ff ff ff ff |... ..@.|........|
|000003a0| 88 22 88 22 88 22 88 22 | 11 22 44 88 11 22 44 88 |."."."."|."D.."D.|
|000003b0| c4 80 0c 68 43 02 30 26 | b1 30 03 1b d8 c0 0c 8d |...hC.0&|.0......|
|000003c0| aa 00 aa 00 aa 00 aa 00 | 88 22 55 22 88 22 55 22 |........|."U"."U"|
|000003d0| 88 55 22 55 88 55 22 55 | 77 dd 77 dd 77 dd 77 dd |.U"U.U"U|w.w.w.w.|
|000003e0| 80 00 00 00 00 00 00 00 | aa 55 aa 55 aa 55 aa 55 |........|.U.U.U.U|
|000003f0| 03 84 48 30 0c 02 01 01 | 82 44 39 44 82 01 01 01 |..H0....|.D9D....|
|00000400| 88 14 22 41 88 41 22 14 | 80 80 41 3e 08 08 14 e3 |.."A.A".|..A>....|
|00000410| 22 04 8c 74 22 17 98 10 | be 80 88 08 eb 08 88 80 |"..t"...|........|
|00000420| 25 c8 32 89 64 24 4c 92 | a2 9c 41 be 2a c9 14 eb |%.2.d$L.|..A.*...|
|00000430| 40 a0 00 00 04 0a 00 00 | 80 40 20 00 02 04 08 00 |@.......|.@ .....|
|00000440| aa 00 80 00 88 00 80 00 | ff 80 80 80 80 80 80 80 |........|........|
|00000450| 08 1c 22 c1 80 01 02 04 | ff 80 80 80 ff 08 08 08 |..".....|........|
|00000460| f8 74 22 47 8f 17 22 71 | bf 00 bf bf b0 b0 b0 b0 |.t"G.."q|........|
|00000470| ff 7f be 5d a2 41 80 00 | fa f5 fa f5 a0 50 a0 50 |...].A..|.....P.P|
|00000480| 00 00 58 c0 00 00 00 40 | 00 00 5d c0 00 00 02 20 |..X....@|..].... |
|00000490| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000004a0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000004b0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000004c0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000004d0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000004e0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000004f0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000500| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000510| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000520| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000530| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000540| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000550| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000560| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000570| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000580| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000590| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000005a0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000005b0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000005c0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000005d0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000005e0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000005f0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000600| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000610| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000620| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000630| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000640| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000650| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000660| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000670| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000680| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000690| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000006a0| 00 20 75 73 69 6e 67 20 | 73 74 61 63 6b 20 22 4c |. using |stack "L|
|000006b0| 61 62 48 65 6c 70 65 72 | 22 0d 69 66 20 69 6e 43 |abHelper|".if inC|
|000006c0| 6f 6c 6f 72 20 69 73 20 | 65 6d 70 74 79 20 74 68 |olor is |empty th|
|000006d0| 65 6e 20 70 75 74 20 74 | 72 75 65 20 69 6e 74 6f |en put t|rue into|
|000006e0| 20 69 6e 43 6f 6c 6f 72 | 0d 63 48 43 73 65 74 55 | inColor|.cHCsetU|
|000006f0| 70 20 69 6e 43 6f 6c 6f | 72 2c 20 46 61 6c 73 65 |p inColo|r, False|
|00000700| 2c 20 22 48 43 63 6f 6c | 6f 72 69 7a 65 22 0d 48 |, "HCcol|orize".H|
|00000710| 43 63 6f 6c 6f 72 69 7a | 65 0d 70 61 73 73 20 6f |Ccoloriz|e.pass o|
|00000720| 70 65 6e 53 74 61 63 6b | 0d 65 6e 64 20 6f 70 65 |penStack|.end ope|
|00000730| 6e 53 74 61 63 6b 0d 00 | 00 00 00 00 00 00 00 00 |nStack..|........|
|00000740| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000750| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000760| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000770| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000780| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000790| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000007a0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000007b0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000007c0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000007d0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000007e0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000007f0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000800| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000810| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000820| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000830| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000840| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000850| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000860| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000870| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000880| 00 00 02 00 4d 41 53 54 | ff ff ff ff 00 00 00 00 |....MAST|........|
|00000890| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000008a0| 00 02 9d ad 00 02 4d 48 | 00 00 94 2e 00 02 18 a0 |......MH|........|
|000008b0| 00 02 c3 c5 00 02 eb 27 | 00 02 c8 c4 00 02 d3 5b |.......'|.......[|
|000008c0| 00 02 b7 71 00 02 2f fe | 00 02 25 e4 00 02 3d 85 |...q../.|..%...=.|
|000008d0| 00 02 0e 76 00 02 55 01 | 00 02 83 9c 00 02 6f d5 |...v..U.|......o.|
|000008e0| 00 00 50 b0 00 00 00 00 | 00 02 d8 5b 00 00 00 00 |..P.....|...[....|
|000008f0| 00 00 54 0d 00 00 00 00 | 00 00 00 00 00 00 00 00 |..T.....|........|
|00000900| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000910| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000920| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000930| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000940| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000950| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000960| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000970| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000980| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000990| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000009a0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000009b0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000009c0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000009d0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000009e0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000009f0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000a00| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000a10| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000a20| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000a30| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000a40| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
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|00000a60| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000a70| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000a80| 00 00 00 80 4c 49 53 54 | 00 00 18 b0 00 00 00 00 |....LIST|........|
|00000a90| 00 00 00 01 00 00 08 00 | 00 00 00 0a 00 30 00 02 |........|.....0..|
|00000aa0| 00 0b 00 03 a0 00 03 8b | 00 00 00 0a 00 00 00 00 |........|........|
|00000ab0| 00 00 1c 0d 00 0a 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000ac0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
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|00000ae0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000af0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000b00| 00 00 08 00 50 41 47 45 | 00 00 1c 0d 00 00 00 00 |....PAGE|........|
|00000b10| 00 00 18 b0 cc 54 b3 3d | 00 00 0b a0 e0 11 09 20 |.....T.=|....... |
|00000b20| 24 02 3c 00 21 1a 8c 14 | 01 0b 8b 00 25 04 89 20 |$.<.!...|....%.. |
|00000b30| 00 c7 18 00 22 11 c9 40 | 7c c0 c2 14 a1 aa 28 a1 |...."..@||.....(.|
|00000b40| 12 c0 21 20 00 2b 32 80 | 00 00 12 e4 a0 08 0c 20 |..! .+2.|....... |
|00000b50| 2d 56 52 00 00 1e 88 24 | 8b 0b 82 00 00 04 11 22 |-VR....$|......."|
|00000b60| 08 85 00 80 a0 10 cd 08 | cc 40 c0 04 83 20 00 81 |........|.@... ..|
|00000b70| 92 84 69 24 88 32 12 00 | 00 00 11 fe a0 42 20 02 |..i$.2..|.....B .|
|00000b80| 64 5d 2a 00 c0 80 b0 04 | 02 1a 03 30 c4 10 51 b0 |d]*.....|...0..Q.|
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|00000ba0| 20 40 5c 20 0a 30 07 00 | 00 00 13 85 b0 00 20 22 | @\ .0..|...... "|
|00000bb0| 20 04 c0 00 c1 84 40 34 | 03 0c 0a 80 08 14 1c 20 | .....@4|....... |
|00000bc0| 04 36 2a 32 80 00 a8 02 | 92 80 00 00 40 00 00 84 |.6*2....|....@...|
|00000bd0| 10 90 44 a1 0c 23 61 00 | 00 00 09 48 b0 00 08 a0 |..D..#a.|...H....|
|00000be0| 84 a6 90 24 01 04 00 16 | 30 0a 0a 08 05 04 1c 20 |...$....|0...... |
|00000bf0| 08 8e 48 40 02 00 89 00 | 94 d0 a0 08 a0 00 20 01 |..H@....|...... .|
|00000c00| 12 08 00 00 00 26 02 00 | 00 00 15 01 b0 13 39 2a |.....&..|......9*|
|00000c10| 76 77 ee 02 f0 ad ff fd | e6 9f fb c9 85 c0 f9 32 |vw......|.......2|
|00000c20| ac ef a3 fe af ab 41 49 | 35 97 b9 39 f7 9e 33 67 |......AI|5..9..3g|
|00000c30| 7e e6 b6 25 0f 05 1e 40 | 00 00 17 d5 b0 01 2a 78 |~..%...@|......*x|
|00000c40| 7c 17 36 00 98 9e ca 24 | 89 59 02 c6 20 34 11 2d ||.6....$|.Y.. 4.-|
|00000c50| 84 3c 18 f0 a0 1a dd 42 | ed c9 40 2d 97 e8 44 84 |.<.....B|..@-..D.|
|00000c60| 14 c2 3b 20 0c 36 1f 40 | 00 00 16 9c a0 79 5c 40 |..; .6.@|.....y\@|
|00000c70| 54 bc 6f 22 e7 45 bf ad | 10 2e 93 00 d7 14 9d 24 |T.o".E..|.......$|
|00000c80| 82 d7 bb 92 a1 4b e8 0e | d9 b0 4e 1c 8a 0a 7a 42 |.....K..|..N...zB|
|00000c90| b4 00 38 a4 4f 3f 67 80 | 00 00 08 ad a0 17 38 a0 |..8.O?g.|......8.|
|00000ca0| 74 47 7c 30 a1 53 8c f4 | 65 29 9b 34 95 54 bb 30 |tG|0.S..|e).4.T.0|
|00000cb0| 82 97 3a 94 ae 49 fb 5a | 3c f1 37 1c b1 ba 28 80 |..:..I.Z|<.7...(.|
|00000cc0| 14 cd b1 10 0d 2b 36 80 | 00 00 10 71 a0 00 08 a1 |.....+6.|...q....|
|00000cd0| 2c 36 10 85 89 1e 88 96 | 49 5b ce 00 05 34 19 a1 |,6......|I[...4..|
|00000ce0| 0a 05 81 a8 20 24 ea 00 | 9c c0 20 24 95 30 00 81 |.... $..|.. $.0..|
|00000cf0| 12 c0 39 10 4c e3 02 00 | 00 00 00 00 00 00 00 00 |..9.L...|........|
|00000d00| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000d10| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
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|00000d40| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
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|00000d60| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000d70| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000d80| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000d90| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000da0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
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|00000dc0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000dd0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000de0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000df0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000e00| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000e10| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000e20| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000e30| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000e40| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000e50| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000e60| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000e70| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000e80| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000e90| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000ea0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000eb0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000ec0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000ed0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000ee0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000ef0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000f00| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000f10| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000f20| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00000f30| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
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|000039a0| 70 65 20 42 6f 6f 6b 20 | 79 6f 75 20 77 61 6e 74 |pe Book |you want|
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|00003d90| 2c 66 61 6c 73 65 2c 22 | 43 68 65 6d 69 63 61 6c |,false,"|Chemical|
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|00003db0| 52 54 46 54 6f 46 69 65 | 6c 64 28 68 6f 6c 64 4e |RTFToFie|ld(holdN|
|00003dc0| 6f 74 65 73 2c 66 61 6c | 73 65 2c 22 4e 6f 74 65 |otes,fal|se,"Note|
|00003dd0| 73 22 29 20 69 6e 74 6f | 20 74 6d 70 0d 70 75 74 |s") into| tmp.put|
|00003de0| 20 68 6f 6c 64 4f 72 69 | 56 6f 6c 20 69 6e 74 6f | holdOri|Vol into|
|00003df0| 20 66 6c 64 20 22 6f 72 | 69 56 6f 6c 22 0d 70 75 | fld "or|iVol".pu|
|00003e00| 74 20 68 6f 6c 64 4f 72 | 69 20 69 6e 74 6f 20 66 |t holdOr|i into f|
|00003e10| 6c 64 20 22 6f 72 69 22 | 0d 70 6f 70 20 63 64 0d |ld "ori"|.pop cd.|
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|00003ed0| 66 20 77 69 6e 64 6f 77 | 20 22 70 72 6f 67 72 65 |f window| "progre|
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|00003f40| 74 6f 20 68 6f 6c 64 4e | 61 6d 65 0d 70 75 74 20 |to holdN|ame.put |
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|00003f80| 75 74 20 46 69 65 6c 64 | 54 6f 52 54 46 28 66 61 |ut Field|ToRTF(fa|
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|00003fb0| 66 6c 64 20 22 6f 72 69 | 56 6f 6c 22 20 69 6e 74 |fld "ori|Vol" int|
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|00004020| 61 72 64 22 0d 70 75 74 | 20 52 54 46 54 6f 46 69 |ard".put| RTFToFi|
|00004030| 65 6c 64 28 68 6f 6c 64 | 4e 61 6d 65 2c 66 61 6c |eld(hold|Name,fal|
|00004040| 73 65 2c 22 4e 61 6d 65 | 22 29 20 69 6e 74 6f 20 |se,"Name|") into |
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|00004060| 6c 64 28 68 6f 6c 64 43 | 68 65 6d 69 63 61 6c 2c |ld(holdC|hemical,|
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|00004090| 54 46 54 6f 46 69 65 6c | 64 28 68 6f 6c 64 4e 6f |TFToFiel|d(holdNo|
|000040a0| 74 65 73 2c 66 61 6c 73 | 65 2c 22 4e 6f 74 65 73 |tes,fals|e,"Notes|
|000040b0| 22 29 20 69 6e 74 6f 20 | 74 6d 70 0d 70 75 74 20 |") into |tmp.put |
|000040c0| 68 6f 6c 64 4f 72 69 56 | 6f 6c 20 69 6e 74 6f 20 |holdOriV|ol into |
|000040d0| 66 6c 64 20 22 6f 72 69 | 56 6f 6c 22 0d 70 75 74 |fld "ori|Vol".put|
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|00004160| 72 6f 6d 4f 74 68 65 72 | 0d 65 6e 64 20 69 66 0d |romOther|.end if.|
|00004170| 67 6f 20 74 6f 20 72 65 | 74 75 72 6e 54 6f 54 68 |go to re|turnToTh|
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|00004190| 6c 6f 63 6b 6d 65 73 73 | 61 67 65 73 20 74 6f 20 |lockmess|ages to |
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|00004210| 0d 65 6e 64 20 69 6d 70 | 6f 72 74 46 72 6f 6d 4f |.end imp|ortFromO|
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|000045e0| 43 20 31 35 20 6d 69 6e | 2e 0d 0d 46 6f 72 20 67 |C 15 min|...For g|
|000045f0| 72 6f 77 69 6e 67 20 73 | 6f 69 6c 20 6d 69 63 72 |rowing s|oil micr|
|00004600| 6f 6f 72 67 61 6e 69 73 | 6d 73 2e 00 00 37 00 02 |oorganis|ms...7..|
|00004610| 00 32 00 36 00 03 00 31 | 30 00 00 31 00 02 00 34 |.2.6...1|0..1...4|
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|00004670| 00 00 00 00 40 00 00 00 | 00 00 00 00 00 00 00 00 |....@...|........|
|00004680| 00 00 1c 0d 00 00 0a 2e | 00 00 00 00 00 00 00 00 |........|........|
|00004690| 00 0a 00 00 01 74 00 01 | 00 0b 00 4d 2d 39 20 70 |.....t..|...M-9 p|
|000046a0| 6c 61 74 65 73 00 00 02 | 00 56 00 4e 61 aa 48 50 |lates...|.V.Na.HP|
|000046b0| 4f a2 0d 4b 48 aa 50 4f | a2 0d 4e 61 43 6c 0d 4e |O..KH.PO|..NaCl.N|
|000046c0| 48 a2 43 6c 0d 61 67 61 | 72 6f 73 65 0d 0d 0d 31 |H.Cl.aga|rose...1|
|000046d0| 20 4d 20 4d 67 53 4f a2 | 0d 31 20 4d 20 43 61 43 | M MgSO.|.1 M CaC|
|000046e0| 6c aa 0d 32 30 20 25 20 | 67 6c 75 63 6f 73 65 0d |l..20 % |glucose.|
|000046f0| 31 20 4d 20 74 68 69 61 | 6d 69 6e 65 2d 48 43 6c |1 M thia|mine-HCl|
|00004700| 00 07 00 05 00 31 30 30 | 30 00 00 05 00 3c 00 36 |.....100|0....<.6|
|00004710| 2e 30 20 67 0d 33 2e 30 | 20 67 0d 30 2e 35 20 67 |.0 g.3.0| g.0.5 g|
|00004720| 0d 31 2e 30 20 67 0d 31 | 35 20 67 0d 0d 0d 32 2e |.1.0 g.1|5 g...2.|
|00004730| 30 20 6d 6c 0d 30 2e 31 | 20 6d 6c 0d 31 30 2e 30 |0 ml.0.1| ml.10.0|
|00004740| 20 6d 6c 0d 31 2e 30 20 | 6d 6c 00 08 00 04 00 32 | ml.1.0 |ml.....2|
|00004750| 35 30 00 11 00 56 00 41 | 64 64 20 48 aa 4f 20 74 |50...V.A|dd H.O t|
|00004760| 6f 20 61 70 70 72 6f 78 | 69 6d 61 74 65 6c 79 20 |o approx|imately |
|00004770| 31 20 6c 69 74 65 72 2e | 20 41 75 74 6f 63 6c 61 |1 liter.| Autocla|
|00004780| 76 65 2e 20 43 6f 6f 6c | 20 74 6f 20 35 30 20 d4 |ve. Cool| to 50 .|
|00004790| 43 2e 20 41 64 64 3a 0d | 0d 0d 0d 66 69 6c 74 65 |C. Add:.|...filte|
|000047a0| 72 20 73 74 65 72 69 6c | 69 7a 65 64 00 37 00 02 |r steril|ized.7..|
|000047b0| 00 33 00 36 00 03 00 31 | 30 00 00 31 00 02 00 34 |.3.6...1|0..1...4|
|000047c0| 00 06 00 45 00 31 2e 35 | 20 67 0d 30 2e 37 35 20 |...E.1.5| g.0.75 |
|000047d0| 67 0d 30 2e 31 32 35 20 | 67 0d 30 2e 32 35 20 67 |g.0.125 |g.0.25 g|
|000047e0| 0d 33 2e 37 35 20 67 0d | 0d 0d 30 2e 35 20 6d 6c |.3.75 g.|..0.5 ml|
|000047f0| 0d 30 2e 30 32 35 20 6d | 6c 0d 32 2e 35 20 6d 6c |.0.025 m|l.2.5 ml|
|00004800| 0d 30 2e 32 35 20 6d 6c | 0d 00 4d 2d 39 20 70 6c |.0.25 ml|..M-9 pl|
|00004810| 61 74 65 73 00 00 00 00 | 00 00 00 00 00 00 00 00 |ates....|........|
|00004820| 00 00 02 00 43 41 52 44 | 00 00 13 85 00 00 00 00 |....CARD|........|
|00004830| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00004840| 00 00 1c 0d 00 00 0a 2e | 00 00 00 00 00 00 00 00 |........|........|
|00004850| 00 0b 00 00 01 ac 00 01 | 00 05 00 4d 53 49 49 00 |........|...MSII.|
|00004860| 00 02 00 6c 00 4d 53 49 | 49 20 28 31 29 3a 0d 4b |...l.MSI|I (1):.K|
|00004870| aa 48 50 4f a2 0d 4b 48 | aa 50 4f a2 0d 4e 48 a2 |.HPO..KH|.PO..NH.|
|00004880| 4e 4f a3 0d 28 4e 48 a2 | 29 a4 4f aa a2 4d 6f a6 |NO..(NH.|).O..Mo.|
|00004890| f9 34 48 aa 4f 20 20 0d | 0d 0d 4d 53 49 49 20 28 |.4H.O .|..MSII (|
|000048a0| 32 29 3a 0d 4d 67 53 4f | a2 f9 37 48 aa 4f 0d 0d |2):.MgSO|..7H.O..|
|000048b0| 0d 4d 53 49 49 20 28 33 | 29 3a 0d 43 61 43 6c aa |.MSII (3|):.CaCl.|
|000048c0| f9 32 48 aa 4f 0d 46 65 | 43 6c a3 f9 36 48 aa 4f |.2H.O.Fe|Cl..6H.O|
|000048d0| 00 11 00 b0 00 40 31 32 | 31 20 d4 43 20 31 35 20 |.....@12|1 .C 15 |
|000048e0| 6d 69 6e 2e 0d 0d 50 72 | 65 70 61 72 65 64 20 61 |min...Pr|epared a|
|000048f0| 73 20 31 30 30 20 6d 6c | 20 61 6c 69 71 75 6f 74 |s 100 ml| aliquot|
|00004900| 2e 0d 0d 55 73 65 20 31 | 30 30 20 6d 6c 20 70 65 |...Use 1|00 ml pe|
|00004910| 72 20 31 20 6c 69 74 65 | 72 20 4d 53 49 49 20 6d |r 1 lite|r MSII m|
|00004920| 65 64 69 75 6d 2e 0d 0d | 0d 66 69 6c 74 72 61 74 |edium...|.filtrat|
|00004930| 69 6f 6e 0d 55 73 65 20 | 31 20 6d 6c 20 70 65 72 |ion.Use |1 ml per|
|00004940| 20 31 20 6c 69 74 65 72 | 20 4d 53 49 49 20 6d 65 | 1 liter| MSII me|
|00004950| 64 69 75 6d 2e 0d 0d 66 | 69 6c 74 72 61 74 69 6f |dium...f|iltratio|
|00004960| 6e 0d 0d 55 73 65 20 31 | 20 6d 6c 20 70 65 72 20 |n..Use 1| ml per |
|00004970| 31 20 6c 69 74 65 72 20 | 4d 53 49 49 20 6d 65 64 |1 liter |MSII med|
|00004980| 69 75 6d 2e 00 05 00 24 | 00 0d 38 20 67 0d 32 0d |ium....$|..8 g.2.|
|00004990| 31 30 0d 30 2e 30 31 0d | 0d 0d 0d 32 30 20 67 0d |10.0.01.|...20 g.|
|000049a0| 0d 0d 0d 32 20 67 0d 30 | 2e 35 20 67 00 07 00 05 |...2 g.0|.5 g....|
|000049b0| 00 31 30 30 30 00 00 08 | 00 04 00 35 30 30 00 37 |.1000...|...500.7|
|000049c0| 00 02 00 34 00 36 00 03 | 00 31 30 00 00 31 00 02 |...4.6..|.10..1..|
|000049d0| 00 34 00 06 00 26 00 0d | 34 20 67 0d 31 0d 35 0d |.4...&..|4 g.1.5.|
|000049e0| 30 2e 30 30 35 0d 0d 0d | 0d 31 30 20 67 0d 0d 0d |0.005...|.10 g...|
|000049f0| 0d 31 20 67 0d 30 2e 32 | 35 20 67 0d 00 32 00 02 |.1 g.0.2|5 g..2..|
|00004a00| 00 31 4d 53 49 49 00 00 | 00 00 00 00 00 00 00 00 |.1MSII..|........|
|00004a10| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00004a20| 00 00 01 00 43 41 52 44 | 00 00 09 48 00 00 00 00 |....CARD|...H....|
|00004a30| 00 00 00 00 40 00 00 00 | 00 00 00 00 00 00 00 00 |....@...|........|
|00004a40| 00 00 1c 0d 00 00 0a 2e | 00 00 00 00 00 00 00 00 |........|........|
|00004a50| 00 09 00 00 00 bc 00 01 | 00 08 00 4e 41 20 26 20 |........|...NA & |
|00004a60| 4e 42 00 02 00 1b 00 62 | 65 65 66 20 65 78 74 72 |NB.....b|eef extr|
|00004a70| 61 63 74 0d 70 65 70 74 | 6f 6e 65 0d 61 67 61 72 |act.pept|one.agar|
|00004a80| 0d 00 00 11 00 56 00 4e | 75 74 72 69 65 6e 74 20 |.....V.N|utrient |
|00004a90| 61 67 61 72 0d 40 31 32 | 31 d4 43 20 31 35 20 6d |agar.@12|1.C 15 m|
|00004aa0| 69 6e 2e 0d 0d 41 54 43 | 43 20 23 33 20 6d 65 64 |in...ATC|C #3 med|
|00004ab0| 69 75 6d 0d 44 49 46 43 | 4f 20 30 39 37 39 0d 0d |ium.DIFC|O 0979..|
|00004ac0| 4e 75 74 72 69 65 6e 74 | 20 62 72 6f 74 68 3a 0d |Nutrient| broth:.|
|00004ad0| 77 69 74 68 6f 75 74 20 | 61 67 61 72 00 07 00 05 |without |agar....|
|00004ae0| 00 31 30 30 30 00 00 05 | 00 0d 00 33 20 67 0d 35 |.1000...|...3 g.5|
|00004af0| 20 67 0d 31 35 20 67 00 | 00 37 00 02 00 35 00 36 | g.15 g.|.7...5.6|
|00004b00| 00 03 00 31 30 00 00 31 | 00 02 00 34 00 32 00 02 |...10..1|...4.2..|
|00004b10| 00 31 4e 41 20 26 20 4e | 42 00 00 00 00 00 00 00 |.1NA & N|B.......|
|00004b20| 00 00 03 40 43 41 52 44 | 00 00 15 01 00 00 00 00 |...@CARD|........|
|00004b30| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00004b40| 00 00 1c 0d 00 00 0a 2e | 00 00 00 00 00 00 00 00 |........|........|
|00004b50| 00 0b 00 00 02 f4 00 01 | 00 14 00 50 43 50 20 49 |........|...PCP I|
|00004b60| 6e 64 69 63 61 74 6f 72 | 20 50 6c 61 74 65 00 07 |ndicator| Plate..|
|00004b70| 00 05 00 31 30 30 30 00 | 00 02 00 6b 00 4b aa 48 |...1000.|...k.K.H|
|00004b80| 50 4f a2 0d 4b 48 aa 50 | 4f a2 0d 4e 48 a2 4e 4f |PO..KH.P|O..NH.NO|
|00004b90| a3 0d 50 43 50 20 28 70 | 48 37 2e 32 29 0d 42 72 |..PCP (p|H7.2).Br|
|00004ba0| 6f 6d 6f 74 68 79 6d 6f | 6c 20 42 6c 75 65 0d 4e |omothymo|l Blue.N|
|00004bb0| 6f 62 6c 65 20 61 67 61 | 72 0d 0d 4d 67 53 4f a2 |oble aga|r..MgSO.|
|00004bc0| f9 37 48 aa 4f 0d 0d 4d | 67 53 4f a2 f9 37 48 aa |.7H.O..M|gSO..7H.|
|00004bd0| 4f 20 35 30 78 20 73 74 | 6f 63 6b 0d 0d 50 43 50 |O 50x st|ock..PCP|
|00004be0| 20 73 74 6f 63 6b 20 00 | 00 05 00 34 00 36 35 20 | stock .|...4.65 |
|00004bf0| 6d 67 0d 31 37 20 6d 67 | 0d 30 2e 35 20 67 0d 30 |mg.17 mg|.0.5 g.0|
|00004c00| 2e 31 20 67 0d 32 30 20 | 6d 67 0d 31 32 20 67 0d |.1 g.20 |mg.12 g.|
|00004c10| 0d 30 2e 31 20 67 0d 0d | 35 20 67 0d 0d 35 20 67 |.0.1 g..|5 g..5 g|
|00004c20| 00 08 00 04 00 32 35 30 | 00 11 01 bc 00 50 6c 61 |.....250|.....Pla|
|00004c30| 74 65 73 20 73 68 6f 75 | 6c 64 20 62 65 20 62 6c |tes shou|ld be bl|
|00004c40| 75 65 20 74 6f 20 73 74 | 61 72 74 20 77 69 74 68 |ue to st|art with|
|00004c50| 2e 20 42 6c 75 65 20 70 | 6c 61 74 65 73 20 77 69 |. Blue p|lates wi|
|00004c60| 6c 6c 20 74 75 72 6e 20 | 79 65 6c 6c 6f 77 20 61 |ll turn |yellow a|
|00004c70| 73 20 43 6c d1 20 61 72 | 65 20 72 65 6c 65 61 73 |s Cl. ar|e releas|
|00004c80| 65 64 20 28 7e 20 70 48 | 20 36 29 2e 0d 0d 41 64 |ed (~ pH| 6)...Ad|
|00004c90| 64 20 4d 67 53 4f a2 20 | 61 66 74 65 72 20 61 75 |d MgSO. |after au|
|00004ca0| 74 6f 63 6c 61 76 69 6e | 67 20 66 72 6f 6d 20 73 |toclavin|g from s|
|00004cb0| 74 65 72 69 6c 65 20 35 | 30 78 20 73 74 6f 63 6b |terile 5|0x stock|
|00004cc0| 0d 28 35 20 67 2f 4c 20 | 73 74 6f 63 6b 3b 20 61 |.(5 g/L |stock; a|
|00004cd0| 64 64 20 32 30 20 6d 6c | 2f 4c 29 0d 0d 44 69 73 |dd 20 ml|/L)..Dis|
|00004ce0| 6f 6c 76 65 20 50 43 50 | 20 69 6e 20 48 aa 4f 20 |olve PCP| in H.O |
|00004cf0| 61 6e 64 20 72 61 69 73 | 65 20 70 48 20 74 6f 20 |and rais|e pH to |
|00004d00| 31 30 20 77 69 74 68 20 | 4e 61 4f 48 2c 20 73 74 |10 with |NaOH, st|
|00004d10| 69 72 20 75 6e 69 74 69 | 6c 20 50 43 50 20 67 6f |ir uniti|l PCP go|
|00004d20| 65 73 20 69 6e 74 6f 20 | 73 6f 6c 6e 2e 20 53 6c |es into |soln. Sl|
|00004d30| 6f 77 6c 79 20 74 61 6b | 65 20 70 48 20 64 6f 77 |owly tak|e pH dow|
|00004d40| 6e 20 74 6f 20 70 48 20 | 37 2e 32 20 28 44 4f 20 |n to pH |7.2 (DO |
|00004d50| 4e 4f 54 20 6f 76 65 72 | 73 68 6f 6f 74 20 6f 74 |NOT over|shoot ot|
|00004d60| 68 65 72 77 69 73 65 20 | 50 43 50 20 77 69 6c 6c |herwise |PCP will|
|00004d70| 20 63 6f 6d 65 20 6f 75 | 74 20 6f 66 20 73 6f 6c | come ou|t of sol|
|00004d80| 75 74 69 6f 6e 29 20 77 | 69 74 68 20 48 aa 53 4f |ution) w|ith H.SO|
|00004d90| a2 20 5b 44 4f 20 4e 4f | 54 20 75 73 65 20 48 43 |. [DO NO|T use HC|
|00004da0| 6c 5d 0d 0d 52 65 66 3a | 20 53 61 62 65 72 20 26 |l]..Ref:| Saber &|
|00004db0| 20 43 72 61 77 66 6f 72 | 2c 20 31 39 38 35 20 41 | Crawfor|, 1985 A|
|00004dc0| 45 4d 20 35 30 3a 20 31 | 35 31 32 20 2d 20 31 35 |EM 50: 1|512 - 15|
|00004dd0| 31 38 20 28 41 70 70 6c | 69 65 64 20 45 6e 76 2e |18 (Appl|ied Env.|
|00004de0| 20 4d 69 63 72 6f 2e 29 | 00 37 00 02 00 36 00 36 | Micro.)|.7...6.6|
|00004df0| 00 03 00 31 30 00 00 31 | 00 02 00 34 00 06 00 44 |...10..1|...4...D|
|00004e00| 00 31 36 2e 32 35 20 6d | 67 0d 34 2e 32 35 20 6d |.16.25 m|g.4.25 m|
|00004e10| 67 0d 30 2e 31 32 35 20 | 67 0d 30 2e 30 32 35 20 |g.0.125 |g.0.025 |
|00004e20| 67 0d 35 20 6d 67 0d 33 | 20 67 0d 0d 30 2e 30 32 |g.5 mg.3| g..0.02|
|00004e30| 35 20 67 0d 0d 31 2e 32 | 35 20 67 0d 0d 31 2e 32 |5 g..1.2|5 g..1.2|
|00004e40| 35 20 67 0d 00 32 00 02 | 00 31 50 43 50 20 49 6e |5 g..2..|.1PCP In|
|00004e50| 64 69 63 61 74 6f 72 20 | 50 6c 61 74 65 00 00 00 |dicator |Plate...|
|00004e60| 00 00 02 80 43 41 52 44 | 00 00 17 d5 00 00 00 00 |....CARD|........|
|00004e70| 00 00 00 00 40 00 00 00 | 00 00 00 00 00 00 00 00 |....@...|........|
|00004e80| 00 00 1c 0d 00 00 0a 2e | 00 00 00 00 00 00 00 00 |........|........|
|00004e90| 00 0b 00 00 02 2a 00 01 | 00 0a 00 53 4f 42 20 26 |.....*..|...SOB &|
|00004ea0| 20 53 4f 43 00 02 00 84 | 00 53 4f 42 3a 0d 62 61 | SOC....|.SOB:.ba|
|00004eb0| 63 74 6f 2d 74 72 79 70 | 74 6f 6e 65 0d 62 61 63 |cto-tryp|tone.bac|
|00004ec0| 74 6f 2d 79 65 61 73 74 | 20 65 78 74 72 61 63 74 |to-yeast| extract|
|00004ed0| 0d 4e 61 43 6c 0d 4b 43 | 6c 0d 0d 53 4f 43 3a 0d |.NaCl.KC|l..SOC:.|
|00004ee0| 53 4f 42 0d 32 20 4d 20 | 4d 67 b1 b1 20 73 74 6f |SOB.2 M |Mg.. sto|
|00004ef0| 63 6b 0d 32 20 4d 20 67 | 6c 75 63 6f 73 65 0d 0d |ck.2 M g|lucose..|
|00004f00| 53 4f 42 3a 0d 62 61 63 | 74 6f 2d 79 65 61 73 74 |SOB:.bac|to-yeast|
|00004f10| 20 65 78 74 72 61 63 74 | 0d 28 6d 6f 64 69 66 69 | extract|.(modifi|
|00004f20| 65 64 20 66 6f 72 20 43 | 52 45 37 29 00 07 00 05 |ed for C|RE7)....|
|00004f30| 00 31 30 30 30 00 00 05 | 00 36 00 0d 32 30 20 67 |.1000...|.6..20 g|
|00004f40| 0d 35 20 67 0d 30 2e 35 | 38 34 20 67 0d 30 2e 31 |.5 g.0.5|84 g.0.1|
|00004f50| 38 36 20 67 0d 0d 0d 39 | 38 30 20 6d 6c 0d 31 30 |86 g...9|80 ml.10|
|00004f60| 20 6d 6c 0d 31 30 20 6d | 6c 0d 0d 0d 31 30 20 67 | ml.10 m|l...10 g|
|00004f70| 00 11 00 ea 00 53 74 65 | 72 69 6c 69 7a 65 20 53 |.....Ste|rilize S|
|00004f80| 4f 42 20 40 31 32 31 d4 | 43 20 66 6f 72 20 32 30 |OB @121.|C for 20|
|00004f90| 20 6d 69 6e 2e 0d 0d 32 | 20 4d 20 4d 67 b1 b1 20 | min...2| M Mg.. |
|00004fa0| 73 74 6f 63 6b 20 61 6e | 64 20 32 20 4d 20 67 6c |stock an|d 2 M gl|
|00004fb0| 75 63 6f 73 65 20 73 68 | 6f 75 6c 64 20 62 65 20 |ucose sh|ould be |
|00004fc0| 66 69 6c 74 65 72 20 73 | 74 65 72 69 6c 69 7a 65 |filter s|terilize|
|00004fd0| 64 2e 0d 0d 46 6f 72 20 | 43 52 45 37 2c 20 74 77 |d...For |CRE7, tw|
|00004fe0| 69 63 65 20 74 68 65 20 | 61 6d 6f 75 6e 74 20 28 |ice the |amount (|
|00004ff0| 31 30 20 67 2f 6c 69 74 | 65 72 29 20 6f 66 20 62 |10 g/lit|er) of b|
|00005000| 61 63 74 6f 2d 79 65 61 | 73 74 20 65 78 74 72 61 |acto-yea|st extra|
|00005010| 63 74 20 69 73 20 61 64 | 64 65 64 20 74 6f 20 70 |ct is ad|ded to p|
|00005020| 72 6f 6c 6f 6e 67 20 74 | 68 65 20 6c 6f 67 20 70 |rolong t|he log p|
|00005030| 68 61 73 65 20 61 6e 64 | 20 72 65 64 75 63 65 20 |hase and| reduce |
|00005040| 74 68 65 20 61 6d 6f 75 | 6e 74 20 6f 66 20 6d 75 |the amou|nt of mu|
|00005050| 63 6f 75 73 20 70 72 6f | 64 75 63 65 64 2e 00 08 |cous pro|duced...|
|00005060| 00 05 00 34 30 30 30 00 | 00 37 00 02 00 37 00 36 |...4000.|.7...7.6|
|00005070| 00 03 00 31 30 00 00 31 | 00 02 00 34 00 32 00 02 |...10..1|...4.2..|
|00005080| 00 31 00 06 00 39 00 0d | 38 30 20 67 0d 32 30 20 |.1...9..|80 g.20 |
|00005090| 67 0d 32 2e 33 33 36 20 | 67 0d 30 2e 37 34 34 20 |g.2.336 |g.0.744 |
|000050a0| 67 0d 0d 0d 33 39 32 30 | 20 6d 6c 0d 34 30 20 6d |g...3920| ml.40 m|
|000050b0| 6c 0d 34 30 20 6d 6c 0d | 0d 0d 34 30 20 67 0d 00 |l.40 ml.|..40 g..|
|000050c0| 53 4f 42 20 26 20 53 4f | 43 00 00 00 00 00 00 00 |SOB & SO|C.......|
|000050d0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000050e0| 00 00 03 40 43 41 52 44 | 00 00 16 9c 00 00 00 00 |...@CARD|........|
|000050f0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00005100| 00 00 1c 0d 00 00 0a 2e | 00 00 00 00 00 00 00 00 |........|........|
|00005110| 00 0a 00 00 02 ce 00 01 | 00 20 00 53 6f 6c 75 74 |........|. .Solut|
|00005120| 69 6f 6e 20 49 2c 20 49 | 49 20 26 20 49 49 49 20 |ion I, I|I & III |
|00005130| 28 41 6c 6b 61 6c 69 6e | 65 29 00 02 00 b9 80 16 |(Alkalin|e)......|
|00005140| 00 00 00 03 00 24 00 01 | 00 25 00 03 00 a0 00 01 |.....$..|.%......|
|00005150| 00 a1 00 03 53 6f 6c 75 | 74 69 6f 6e 20 49 3a 0d |....Solu|tion I:.|
|00005160| 35 30 20 6d 4d 20 67 6c | 75 63 6f 73 65 0d 32 35 |50 mM gl|ucose.25|
|00005170| 20 6d 4d 20 54 72 69 73 | f9 43 6c 20 28 70 48 20 | mM Tris|.Cl (pH |
|00005180| 38 2e 30 29 0d 31 30 20 | 6d 4d 20 45 44 54 41 20 |8.0).10 |mM EDTA |
|00005190| 28 70 48 20 38 2e 30 29 | 0d 0d 53 6f 6c 75 74 69 |(pH 8.0)|..Soluti|
|000051a0| 6f 6e 20 49 49 3a 0d 30 | 2e 32 20 4e 20 4e 61 4f |on II:.0|.2 N NaO|
|000051b0| 48 0d 31 20 25 20 53 44 | 53 0d 0d 53 6f 6c 75 74 |H.1 % SD|S..Solut|
|000051c0| 69 6f 6e 20 49 49 49 3a | 0d 35 20 4d 20 70 6f 74 |ion III:|.5 M pot|
|000051d0| 61 73 73 69 75 6d 20 61 | 63 65 74 61 74 65 0d 67 |assium a|cetate.g|
|000051e0| 6c 61 63 69 61 6c 20 61 | 63 65 74 69 63 20 61 63 |lacial a|cetic ac|
|000051f0| 69 64 0d 48 aa 4f 0d 00 | 00 05 00 3f 00 0d 34 2e |id.H.O..|...?..4.|
|00005200| 35 30 34 20 67 0d 31 2e | 39 37 20 67 0d 31 2e 34 |504 g.1.|97 g.1.4|
|00005210| 36 31 32 20 67 0d 0d 0d | 34 2e 30 30 31 20 67 0d |612 g...|4.001 g.|
|00005220| 35 20 67 0d 0d 0d 36 30 | 20 6d 6c 0d 31 31 2e 35 |5 g...60| ml.11.5|
|00005230| 20 6d 6c 0d 32 38 2e 35 | 20 6d 6c 00 00 07 00 04 | ml.28.5| ml.....|
|00005240| 00 35 30 30 00 11 01 3c | 80 16 00 00 00 03 00 fc |.500...<|........|
|00005250| 00 01 01 09 00 03 01 18 | 00 01 01 25 00 03 53 6f |........|...%..So|
|00005260| 6c 75 74 69 6f 6e 20 49 | 20 63 61 6e 20 62 65 20 |lution I| can be |
|00005270| 70 72 65 70 61 72 65 64 | 20 69 6e 20 62 61 74 63 |prepared| in batc|
|00005280| 68 65 73 20 6f 66 20 7e | 20 31 30 30 20 6d 6c 2c |hes of ~| 100 ml,|
|00005290| 20 61 75 74 6f 63 6c 61 | 76 65 64 20 66 6f 72 20 | autocla|ved for |
|000052a0| 31 35 20 6d 69 6e 75 74 | 65 73 20 61 74 20 31 30 |15 minut|es at 10|
|000052b0| 20 6c 62 2f 73 71 2e 20 | 69 6e 2e 20 6f 6e 20 6c | lb/sq. |in. on l|
|000052c0| 69 71 75 69 64 20 63 79 | 63 6c 65 20 61 6e 64 20 |iquid cy|cle and |
|000052d0| 73 74 6f 72 65 64 20 61 | 74 20 34 20 a1 43 2e 0d |stored a|t 4 .C..|
|000052e0| 0d 41 64 64 20 61 62 6f | 75 74 20 34 20 74 61 62 |.Add abo|ut 4 tab|
|000052f0| 6c 65 74 20 6f 66 20 4e | 61 4f 48 20 63 72 79 73 |let of N|aOH crys|
|00005300| 74 61 6c 20 74 6f 20 61 | 64 6a 75 73 74 20 74 68 |tal to a|djust th|
|00005310| 65 20 70 48 20 74 6f 20 | 38 2e 30 2e 0d 0d 53 6f |e pH to |8.0...So|
|00005320| 6c 75 74 69 6f 6e 20 49 | 49 0d 30 2e 32 20 4e 20 |lution I|I.0.2 N |
|00005330| 4e 61 4f 48 20 28 66 72 | 65 73 68 6c 79 20 64 69 |NaOH (fr|eshly di|
|00005340| 6c 75 74 65 64 20 66 72 | 6f 6d 20 61 20 31 30 20 |luted fr|om a 10 |
|00005350| 4e 20 73 74 6f 63 6b 29 | 0d 0d 40 31 32 31 d4 43 |N stock)|..@121.C|
|00005360| 20 31 35 20 6d 69 6e 0d | 0d 53 6f 6c 75 74 69 6f | 15 min.|.Solutio|
|00005370| 6e 20 49 49 49 0d 40 31 | 32 31 d4 43 20 31 35 20 |n III.@1|21.C 15 |
|00005380| 6d 69 6e 0d 00 08 00 05 | 00 31 30 30 30 00 00 37 |min.....|.1000..7|
|00005390| 00 02 00 38 00 36 00 03 | 00 31 30 00 00 31 00 02 |...8.6..|.10..1..|
|000053a0| 00 34 00 06 00 3d 00 0d | 39 2e 30 30 38 20 67 0d |.4...=..|9.008 g.|
|000053b0| 33 2e 39 34 20 67 0d 32 | 2e 39 32 32 20 67 0d 0d |3.94 g.2|.922 g..|
|000053c0| 0d 38 2e 30 30 32 20 67 | 0d 31 30 20 67 0d 0d 0d |.8.002 g|.10 g...|
|000053d0| 31 32 30 20 6d 6c 0d 32 | 33 20 6d 6c 0d 35 37 20 |120 ml.2|3 ml.57 |
|000053e0| 6d 6c 0d 00 53 6f 6c 75 | 74 69 6f 6e 20 49 2c 20 |ml..Solu|tion I, |
|000053f0| 49 49 20 26 20 49 49 49 | 20 28 41 6c 6b 61 6c 69 |II & III| (Alkali|
|00005400| 6e 65 29 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |ne).....|........|
|00005410| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00005420| 00 00 03 40 43 41 52 44 | 00 00 08 ad 00 00 00 00 |...@CARD|........|
|00005430| 00 00 00 00 40 00 00 00 | 00 00 00 00 00 00 00 00 |....@...|........|
|00005440| 00 00 1c 0d 00 00 0a 2e | 00 00 00 00 00 00 00 00 |........|........|
|00005450| 00 0a 00 00 02 e8 00 01 | 00 14 00 54 42 20 28 54 |........|...TB (T|
|00005460| 65 72 72 69 66 69 63 20 | 42 72 6f 74 68 29 00 02 |errific |Broth)..|
|00005470| 00 3d 00 62 61 63 74 6f | 2d 74 72 79 70 74 6f 6e |.=.bacto|-trypton|
|00005480| 65 0d 62 61 63 74 6f 2d | 79 65 61 73 74 20 65 78 |e.bacto-|yeast ex|
|00005490| 74 72 61 63 74 0d 67 6c | 79 63 65 72 6f 6c 0d 0d |tract.gl|ycerol..|
|000054a0| 0d 4b 48 aa 50 4f a2 0d | 4b aa 48 50 4f a2 0d 00 |.KH.PO..|K.HPO...|
|000054b0| 00 11 02 16 00 46 6f 72 | 20 6f 6e 65 20 6c 69 74 |.....For| one lit|
|000054c0| 65 72 20 6f 66 20 54 42 | 20 6d 65 64 69 75 6d 2c |er of TB| medium,|
|000054d0| 20 64 69 73 73 6f 6c 76 | 65 64 20 69 6e 20 39 30 | dissolv|ed in 90|
|000054e0| 30 20 6d 6c 20 77 61 74 | 65 72 20 66 69 72 73 74 |0 ml wat|er first|
|000054f0| 2e 20 0d 0d 53 74 65 72 | 69 6c 69 7a 65 20 62 79 |. ..Ster|ilize by|
|00005500| 20 61 75 74 6f 63 6c 61 | 76 69 6e 67 20 66 6f 72 | autocla|ving for|
|00005510| 20 32 30 20 6d 69 6e 75 | 74 65 73 20 61 74 20 31 | 20 minu|tes at 1|
|00005520| 35 20 6c 62 2f 73 71 2e | 20 69 6e 2e 20 6f 6e 20 |5 lb/sq.| in. on |
|00005530| 6c 69 71 75 69 64 20 63 | 79 63 6c 65 2e 0d 0d 41 |liquid c|ycle...A|
|00005540| 6c 6c 6f 77 20 74 68 65 | 20 73 6f 6c 75 74 69 6f |llow the| solutio|
|00005550| 6e 20 74 6f 20 63 6f 6f | 6c 20 74 6f 20 36 30 d4 |n to coo|l to 60.|
|00005560| 43 20 6f 72 20 6c 65 73 | 73 2c 20 61 6e 64 20 74 |C or les|s, and t|
|00005570| 68 65 6e 20 61 64 64 20 | 31 30 30 20 6d 6c 20 6f |hen add |100 ml o|
|00005580| 66 20 61 20 73 74 65 72 | 69 6c 65 20 73 6f 6c 75 |f a ster|ile solu|
|00005590| 74 69 6f 6e 20 6f 66 20 | 30 2e 31 37 20 4d 20 4b |tion of |0.17 M K|
|000055a0| 48 aa 50 4f a2 2c 20 30 | 2e 37 32 20 4d 20 4b aa |H.PO., 0|.72 M K.|
|000055b0| 48 50 4f a2 2e 20 28 54 | 68 69 73 20 73 6f 6c 75 |HPO.. (T|his solu|
|000055c0| 74 69 6f 6e 20 69 73 20 | 6d 61 64 65 20 62 79 20 |tion is |made by |
|000055d0| 64 69 73 73 6f 6c 76 69 | 6e 67 20 32 2e 33 31 20 |dissolvi|ng 2.31 |
|000055e0| 67 20 6f 66 20 4b 48 aa | 50 4f a2 20 61 6e 64 20 |g of KH.|PO. and |
|000055f0| 31 32 2e 35 34 20 67 20 | 6f 66 20 4b aa 48 50 4f |12.54 g |of K.HPO|
|00005600| a2 20 69 6e 20 39 30 20 | 6d 6c 20 6f 66 20 64 65 |. in 90 |ml of de|
|00005610| 69 6f 6e 69 7a 65 64 20 | 48 aa 4f 2e 20 41 66 74 |ionized |H.O. Aft|
|00005620| 65 72 20 74 68 65 20 73 | 61 6c 74 73 20 68 61 76 |er the s|alts hav|
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|00005650| 66 20 74 68 65 20 73 6f | 6c 75 74 69 6f 6e 20 74 |f the so|lution t|
|00005660| 6f 20 31 30 30 20 6d 6c | 20 77 69 74 68 20 64 65 |o 100 ml| with de|
|00005670| 69 6f 6e 69 7a 65 64 20 | 48 aa 4f 20 61 6e 64 20 |ionized |H.O and |
|00005680| 73 74 65 72 69 6c 69 7a | 65 20 62 79 20 61 75 74 |steriliz|e by aut|
|00005690| 6f 63 6c 61 76 69 6e 67 | 20 66 6f 72 20 32 30 20 |oclaving| for 20 |
|000056a0| 6d 69 6e 75 74 65 73 20 | 61 74 20 31 35 20 6c 62 |minutes |at 15 lb|
|000056b0| 2f 73 71 2e 20 69 6e 2e | 20 6f 6e 20 6c 69 71 75 |/sq. in.| on liqu|
|000056c0| 69 64 20 63 79 63 6c 65 | 2e 29 00 07 00 05 00 31 |id cycle|.).....1|
|000056d0| 30 30 30 00 00 05 00 20 | 00 31 32 20 67 0d 32 34 |000.... |.12 g.24|
|000056e0| 20 67 0d 34 20 6d 6c 0d | 0d 0d 32 2e 33 31 20 67 | g.4 ml.|..2.31 g|
|000056f0| 0d 31 32 2e 35 34 20 67 | 00 08 00 05 00 35 30 30 |.12.54 g|.....500|
|00005700| 30 00 00 06 00 23 00 36 | 30 20 67 0d 31 32 30 20 |0....#.6|0 g.120 |
|00005710| 67 0d 32 30 20 6d 6c 0d | 0d 0d 31 31 2e 35 35 20 |g.20 ml.|..11.55 |
|00005720| 67 0d 36 32 2e 37 20 67 | 0d 00 00 37 00 02 00 39 |g.62.7 g|...7...9|
|00005730| 00 36 00 03 00 31 30 00 | 00 31 00 02 00 34 54 42 |.6...10.|.1...4TB|
|00005740| 20 28 54 65 72 72 69 66 | 69 63 20 42 72 6f 74 68 | (Terrif|ic Broth|
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|00005790| 00 0a 00 00 01 2e 00 01 | 00 04 00 54 59 45 00 02 |........|...TYE..|
|000057a0| 00 42 00 62 61 63 74 6f | 2d 74 72 79 70 74 6f 6e |.B.bacto|-trypton|
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|000057d0| 72 20 28 66 6f 72 20 73 | 6f 6c 69 64 20 6d 65 64 |r (for s|olid med|
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|000057f0| 00 13 00 35 20 67 0d 32 | 2e 35 20 67 0d 31 20 67 |...5 g.2|.5 g.1 g|
|00005800| 0d 31 35 20 67 00 00 11 | 00 7e 00 40 31 32 31 d4 |.15 g...|.~.@121.|
|00005810| 43 20 31 35 20 6d 69 6e | 0d 54 68 69 73 20 6d 65 |C 15 min|.This me|
|00005820| 64 69 75 6d 20 69 73 20 | 69 64 65 61 6c 20 66 6f |dium is |ideal fo|
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|000058a0| 00 04 00 31 30 30 00 06 | 00 1a 00 30 2e 35 20 67 |...100..|...0.5 g|
|000058b0| 0d 30 2e 32 35 20 67 0d | 30 2e 31 20 67 0d 31 2e |.0.25 g.|0.1 g.1.|
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|00005d80| 00 05 00 00 ff ff 66 69 | 65 6c 64 20 22 66 69 6e |......fi|eld "fin|
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|00005de0| 00 00 00 60 46 54 42 4c | 00 00 0d 27 00 00 00 00 |...`FTBL|...'....|
|00005df0| 00 00 00 05 00 00 00 00 | 00 00 43 68 69 63 61 67 |........|..Chicag|
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|00005e30| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00005e40| 00 00 02 20 46 52 45 45 | 00 00 00 00 00 00 00 00 |... FREE|........|
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